Antioxidant Enzyme Activities, Metallothioneins and Lipid Peroxidation as Biomarkers in Ruditapes decussatus ?

作者: Florence Geret , Angela Serafim , Maria João Bebianno

DOI: 10.1023/A:1026108306755

关键词: CatalaseEnvironmental pollutionSuperoxide dismutaseBiochemistryBivalviaGlutathione peroxidaseAntioxidantLipid peroxidationBiologyGill

摘要: Antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPX)) were studied in the gills and digestive gland of clams Ruditapes decussatus from different sites Ria Formosa (south coast Portugal) order to validate these as biomarkers metal exposure. Two other potential biomarkers, lipid peroxidation expressed malondialdehyde (MDA), metallothionein (MT) levels also measured. This study demonstrates that mitochondrial cytosolic SOD activity changed while CAT (cytosolic mitochondrial) was higher three affected by sources contamination. GPXs (total Se-dependent) inhibited A (directly influenced sewage industrial wastes) B harbor facilities). MT MDA two third site (C, main navigation channel exchange with Atlantic ocean). These results suggest GPX activities especially can be used for monitoring environmental pollution.

参考文章(33)
Quig D, Cysteine metabolism and metal toxicity. Alternative medicine review : a journal of clinical therapeutic. ,vol. 3, pp. 262- 270 ,(1998)
Amaia Orbea, H. Dariush Fahimi, Miren P. Cajaraville, Immunolocalization of four antioxidant enzymes in digestive glands of mollusks and crustaceans and fish liver Histochemistry and Cell Biology. ,vol. 114, pp. 393- 404 ,(2000) , 10.1007/S004180000207
B Burlando, I Panfoli, A Viarengo, N Ceratto, Antioxidant role of metallothioneins: a comparative overview. Cellular and Molecular Biology. ,vol. 46, pp. 407- 417 ,(2000)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Joe M. McCord, Irwin Fridovich, Superoxide Dismutase AN ENZYMIC FUNCTION FOR ERYTHROCUPREIN (HEMOCUPREIN) Journal of Biological Chemistry. ,vol. 244, pp. 6049- 6055 ,(1969) , 10.1016/S0021-9258(18)63504-5
F. Regoli, H. Hummel, C. Amiard-Triquet, C. Larroux, A. Sukhotin, Trace metals and variations of antioxidant enzymes in Arctic bivalve populations. Archives of Environmental Contamination and Toxicology. ,vol. 35, pp. 594- 601 ,(1998) , 10.1007/S002449900421
C. Porte, M. Sole, J. Albaigés, D.R. Livingstone, Responses of mixed-function oxygenase and antioxidase enzyme system of Mytilus sp. to organic pollution Comparative Biochemistry and Physiology Part C: Comparative Pharmacology. ,vol. 100, pp. 183- 186 ,(1991) , 10.1016/0742-8413(91)90150-R
Irene Erdelmeier, Dominique Gérard-Monnier, Jean-Claude Yadan, Jean Chaudière, Reactions of N-Methyl-2-phenylindole with Malondialdehyde and 4-Hydroxyalkenals. Mechanistic Aspects of the Colorimetric Assay of Lipid Peroxidation Chemical Research in Toxicology. ,vol. 11, pp. 1184- 1194 ,(1998) , 10.1021/TX970180Z
Richard A. Lawrence, Raymond F. Burk, Glutathione peroxidase activity in selenium-deficient rat liver Biochemical and Biophysical Research Communications. ,vol. 71, pp. 952- 958 ,(1976) , 10.1016/0006-291X(76)90747-6