X-Ray Spectroscopy in the Clinical Laboratory III. Sulfur Distribution in the Electrophoretic Protein Fractions of Human Serum; Abnormalities Observed in Certain Disease States

摘要: Sulfur analysis in serum is performed with the X-ray spectrometer. The total sulfur content of normal healthy adults found to be 98 mg./100 ml. ± 2.0 (2σ). This calculates 1.44 gm. per 100 proteins 0.1 distribution paper electrophoretic fractions was assayed by performing electrophoresis and passing unstained through field at a constant velocity; 69.3 cent albumin fraction. other contain, for alpha 1 , 2 beta, gamma globulins, 4.2, 9.5, 7.5 cent, respectively, as mean values 16 adults. From protein each fraction, determined staining, calculated fraction follows: Albumin, 1.74; A,, 1.61; A2, 1.37; 1.09; gamma, 0.67. from two sera containing an abnormal migrating globulins studied. low these patients—only 0.18 0.16 being found—as compared 0.67 normal. Both came patients multiple myeloma. In patients, which had traveling beta one contained level elevated 1.42 gm./100 protein, 1.09 globulin. diagnosis this case second low-0.43 protein. patient equivocal. Technics are described determining different