Heterologous expression of ACC deaminase from Trichoderma asperellum improves the growth performance of Arabidopsis thaliana under normal and salt stress conditions.

摘要: Transgenic Arabidopsis thaliana plants expressing the 1-aminocyclopropane-1-carboxylate deaminase gene (ACCD) of Trichoderma asperellum ACCC30536 (TaACCD) were created and their growth performance was assessed under normal salt stress conditions. In order to characterize growth, root length, number, fresh weight (FW), relative water content (RWC), seed production, number measured. Under growing condition, all parameters except for dry (DW) transgenic increased significantly compared WT plants. Furthermore, line also exhibited higher tolerance faster than in presence 150 mM NaCl. The is attributed a greater RWC, weight, FW stress, reduced reactive oxygen species (ROS) level, cell death electrolyte leakage reduction ROS levels could be explained by activity several antioxidant enzymes, including peroxidase (POD), superoxide dismutase (SOD), catalase (CAT). Thus, we propose that heterologous expression TaACCD used improve