Performance and specificity of the covalently linked immunomagnetic separation-ATP method for rapid detection and enumeration of enterococci in coastal environments.

摘要: The performance and specificity of the covalently linked immunomagnetic separation-ATP (Cov-IMS/ATP) method for detection enumeration enterococci was evaluated in recreational waters. Cov-IMS/ATP compared with standard methods: defined substrate technology (Enterolert; IDEXX Laboratories), membrane filtration (EPA Method 1600), an Enterococcus-specific quantitative PCR (qPCR) assay A). We extend previous studies by (i) analyzing stability relationship between culture-based methods at different field sites, (ii) evaluating seven ATCC Enterococcus species, (iii) identifying cross-reacting organisms binding antibody-bead complexes 16S rRNA gene sequencing to five nonenterococcus (iv) conducting preliminary tests preabsorption as a means improving assay. found perform consistently strong agreement rates (based on exceedance/compliance regulatory limits) 83% 100% Enterolert variety sites complex inputs. is specific spp. tested. However, there potential nontarget bacteria bind antibody, which may be reduced purification IgG serum problematic sites. findings this study help validate method, suggesting predictable traditional methods, will allow more widespread application rapid field-portable coastal water quality assessment.