Malate and Fumarate Emerge as Key Players in Primary Metabolism: Arabidopsis thaliana Overexpressing C4-NADP-ME Offer a Way to Manipulate the Levels of Malate and to Analyse the Physiological Consequences

摘要: Maize C4-NADP-malic enzyme was expressed under the control of CaMV 35S promoter in Arabidopsis thaliana. An increase plastidic NADP-ME activity induced no phenotypic differences long-day growth conditions. Analysis metabolite levels, however, revealed a disturbed metabolic profile. Dark-induced senescence progressed more rapidly MEm plants compared to wild-type. A retardation transgenic lines gained by exogenous supply glucose, sucrose and malate, suggesting that lack rapid energy source is likely be initial factor leading induction these plants. fairly complete picture primary metabolism assessed GC-MS vitro complementation assays allow us conclude entered dark due an accelerated starvation. Comparison data obtained indicated extremely low levels malate fumarate are responsible for dark-induced encountered Reinforcing previous results, our indicate key players