Identification of TLR10 as a Key Mediator of the Inflammatory Response toListeria monocytogenesin Intestinal Epithelial Cells and Macrophages

摘要: Listeria monocytogenes is a Gram-positive bacterium that can cause septicemia and meningitis. TLRs are central receptors of the innate immune system drive inflammatory responses to invading microbes such as L. monocytogenes. Although intestinal epithelial cells (IECs) represent initial point entry used by for infection, response in these has been poorly characterized date. The aim this study was determine which involved mediating IECs. We performed an RNA interference screen 1-10 HT-29 IEC cell line observed most significant reduction chemokine output following silencing TLR10. This effect also macrophage THP-1. chemokines CCL20, CCL1, IL-8 were reduced knockdown Silencing TLR10 resulted increased viability both THP-1 cells. found be predominantly expressed intracellularly epithelia, activation required viable NF-κB seen require TLR2 addition Taken together, data indicate novel roles sensing pathogenic infection epithelium macrophages have identified source ligand orphan receptor