A proteomic approach to analysing responses of Arabidopsis thaliana callus cells to clinostat rotation

摘要: Callus cells of Arabidopsis thaliana (cv. Landsberg erecta) were exposed for 8 h to a horizontal clinostat rotation (H, simulated weightlessness), vertical (V, control), or stationary control (S) growth condition. The amount glucose and fructose apparently decreased, while starch content increased in the H compared with V- S-treated cells. In order investigate influences on cellular proteome further, alterations induced by have been comparatively analysed highresolution two-dimensional (2-D) gel electrophoresis mass spectrometry. Image analysis silverstained 2-D gels revealed that 80 protein spots showed quantitative qualitative variations significantly (P <0.01) reproducibly different between clinorotated (H V) samples. Protein excised from microbe high performance liquid chromatography-ion trap-mass spectrometry (LC-IT-MS) obtain tandem (MS/MS) spectra. 18 spots, which significant expression alteration only under condition those V S conditions, identified. Of these proteins, seven involved stress responses, four identified as key enzymes carbohydrate metabolism lipid biosynthesis. Two reversibly glycosylated cell wall proteins down-regulated Other such disulphide isomerase, transcription initiation factor IIF, two ribosomal also exhibited altered data presented this study illustrate callus has impact general metabolic pathways, gene activation/transcription, synthesis,