Characterization and transcriptional analyses of cDNAs encoding three trypsin‐ and chymotrypsin‐like proteinases in Cry1Ab‐susceptible and Cry1Ab‐resistant strains of sugarcane borer, Diatraea saccharalis
作者： Yunlong Yang , Yu Cheng Zhu , James Ottea , Claudia Husseneder , B Rogers Leonard
DOI: 10.1111/J.1744-7917.2012.01514.X
关键词: Diatraea saccharalis 、 Chymotrypsin 、 Signal peptide 、 Biology 、 Catalytic triad 、 Amino acid 、 Biochemistry 、 Trypsin 、 Complementary DNA 、 Midgut
摘要: Diatraea saccharalis is a major corn borer pest. Midgut serine proteinases are essential for insect growth and development. Alteration of midgut responsible Bt resistance development in some species. To clone trypsin chymotrypsin cDNAs to test if the Cry1Ab D. associated with changes proteinases, total tryptic chymotryptic activities, cDNA sequences, gene expressions three genes were comparatively examined between Cry1Ab-susceptible (Cry1Ab-SS) Cry1Ab-resistant (Cry1Ab-RR) strains. Full-length encoding trypsin- chymotrypsin-like sequenced from Cry1Ab-SS Cry1Ab-RR larvae. These code active forms all functional motifs, including signal peptide, conserved His-Asp-Ser catalytic triad, pairs cysteines disulfide bridge configurations, substrate specificity determination residues. In general, putative protein sequences highly similar strains, except few nucleotide predicted amino acid substitutions, whose function need be further clarified. Total activities also Transcriptional levels had numerical difference but was not statistically significant. Data suggest that significantly these trypsins chymotrypsins. Results clarified role six provided foundation continuing examination potential involvement other important biochemical processes.
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sci-hub.se 参考文章(45)
Y ZHU, X LIU, A MADDUR, B OPPERT, M CHEN, Cloning and characterization of chymotrypsin- and trypsin-like cDNAs from the gut of the Hessian fly [Mayetiola destructor (say)] Insect Biochemistry and Molecular Biology. ,vol. 35, pp. 23- 32 ,(2005) , 10.1016/J.IBMB.2004.09.006
Walter R. Terra, Clélia Ferreira, Insect digestive enzymes: properties, compartmentalization and function Comparative Biochemistry and Physiology B. ,vol. 109, pp. 1- 62 ,(1994) , 10.1016/0305-0491(94)90141-4
Michael G. Koziel, Gary L. Beland, Cindy Bowman, Nadine B. Carozzi, Rebecca Crenshaw, Lyle Crossland, John Dawson, Nalini Desai, Martha Hill, Sue Kadwell, Karen Launis, Kelly Lewis, Daryl Maddox, Kathryn McPherson, Moez R. Meghji, Ellis Merlin, Richard Rhodes, Gregory W. Warren, Martha Wright, Stephen V. Evola, Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis Nature Biotechnology. ,vol. 11, pp. 194- 200 ,(1993) , 10.1038/NBT0293-194
Laurence N Gatehouse, April L. Shannon, Elisabeth P.J Burgess, John T Christeller, Characterization of major midgut proteinase cDNAs from Helicoverpa armigera larvae and changes in gene expression in response to four proteinase inhibitors in the diet Insect Biochemistry and Molecular Biology. ,vol. 27, pp. 929- 944 ,(1997) , 10.1016/S0965-1748(97)00074-X
Shaojiu Wang, Felix Young, Donal A. Hickey, Genomic organization and expression of a trypsin gene from the spruce budworm, Choristoneura fumiferana Insect Biochemistry and Molecular Biology. ,vol. 25, pp. 899- 908 ,(1995) , 10.1016/0965-1748(95)00022-N
R. Milne, H. Kaplan, Purification and characterization of a trypsin-like digestive enzyme from spruce budworm (Chroristoneura fumiferana) responsible for the activation of δ-endotoxin from Bacillus thuringiensis Insect Biochemistry and Molecular Biology. ,vol. 23, pp. 663- 673 ,(1993) , 10.1016/0965-1748(93)90040-Y
Carmen Forcada, Encarna Alcácer, M. Dolores Garcerá, Rafael Martínez, DIFFERENCES IN THE MIDGUT PROTEOLYTIC ACTIVITY OF TWO HELIOTHIS VIRESCENS STRAINS, ONE SUSCEPTIBLE AND ONE RESISTANT TO BACILLUS THURINGIENSIS TOXINS Archives of Insect Biochemistry and Physiology. ,vol. 31, pp. 257- 272 ,(1996) , 10.1002/(SICI)1520-6327(1996)31:3<257::AID-ARCH2>3.0.CO;2-V
David P Bown, Hillary S Wilkinson, John A Gatehouse, Differentially regulated inhibitor-sensitive and insensitive protease genes from the phytophagous insect pest, Helicoverpa armigera, are members of complex multigene families Insect Biochemistry and Molecular Biology. ,vol. 27, pp. 625- 638 ,(1997) , 10.1016/S0965-1748(97)00043-X
Fangneng Huang, Roy Parker, Rogers Leonard, Yunlong Yong, Jin Liu, Frequency of resistance alleles to Bacillus thuringiensis-corn in Texas populations of the sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae). Crop Protection. ,vol. 28, pp. 174- 180 ,(2009) , 10.1016/J.CROPRO.2008.10.002
Yunlong Yang, Yu Cheng Zhu, James Ottea, Claudia Husseneder, B. Rogers Leonard, Craig Abel, Fangneng Huang, Molecular characterization and RNA interference of three midgut aminopeptidase N isozymes from Bacillus thuringiensis-susceptible and -resistant strains of sugarcane borer, Diatraea saccharalis. Insect Biochemistry and Molecular Biology. ,vol. 40, pp. 592- 603 ,(2010) , 10.1016/J.IBMB.2010.05.006