Ground-state-depletion fluorscence microscopy: A concept for breaking the diffraction resolution limit

摘要: We introduce and study a novel concept in farfield fluorescence microscopy fundamentally overcoming the classical diffraction resolution limit. This is accomlished by reducing spatial extent of effective focus scanning microscope. The reduction achieved depleting ground-state energy molecules located outer region focus. Our theoretical shows that ground-state-depletion has potential increasing far-field an order magnitude which equivalent to lateral 15 NM.