Quantitative real-time RT-PCR analysis of inflammatory gene expression associated with ischemia-reperfusion brain injury.

摘要: Ischemia-reperfusion brain injury initiates an inflammatory response involving the expression of adhesion molecules and cytokines, some which are regulated by nuclear transcription factor NF-kappaB. In this study authors examined mRNA levels for several important genes associated with inflammation at five time points (3, 6, 12, 24, 72 hours) after transient middle cerebral artery occlusion (MCAO) in Sprague-Dawley rats. A sensitive quantitative technique (TaqMan real-time QRT-PCR) was used to simultaneously measure key cell cytokines. Gene increased significantly injured hemisphere interleukin (IL)-1beta (12-fold increase 24 hours), IL-6 (25-fold 6 ICAM-1 (4-fold interhemispheric differences these were significant every point (P < 0.05 all values). Tumor necrosis factor-alpha upregulated versus uninjured from 3 hours (5-fold while E-selectin showed a MCAO (10-fold VCAM-1 did not respond differentially any between two hemispheres. At examined, activated NF-kappaB immunoreactivity observed cells throughout infarct-damaged tissue. These results consistent proinflammatory properties induced molecules, involved initiation cascade, may thus contribute secondary cellular responses that lead further damage.