Labeling of HeLa cells using ZrO2:Yb3+-Er3+ nanoparticles with upconversion emission
作者： Andrea Ceja-Fdez , Tzarara López-Luke , Jorge Oliva , Juan Vivero-Escoto , Ana Lilia Gonzalez-Yebra
DOI: 10.1117/1.JBO.20.4.046006
关键词: Photoluminescence 、 Triethoxysilane 、 Zeta potential 、 Chemistry 、 Photon upconversion 、 Photochemistry 、 Confocal microscopy 、 Nanoparticle 、 Dynamic light scattering 、 Luminescence 、 Nanotechnology
摘要: This work reports the synthesis, structural characterization, and optical properties of ZrO2:Yb3+-Er3+ (2–1 mol%) nanocrystals. The nanoparticles were coated with 3-aminopropyl triethoxysilane (APTES) further modified biomolecules, such as Biotin-Anti-rabbit (mouse IgG) rabbit antibody-AntiKi-67, through a conjugation method. was successfully confirmed by Fourier transform infrared, zeta potential, dynamic light scattering. internalization conjugated in human cervical cancer (HeLa) cells followed two-photon confocal microscopy. nanocrystals exhibited strong red emission under 970-nm excitation. Moreover, luminescence change due to addition APTES molecules biomolecules on also studied. These results demonstrate that can be functionalized develop platforms for biolabeling bioimaging.
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sci-hub.se 参考文章(70)
Fernando Guerra, Adriana Esther Rocher, José Villacorta Hidalgo, Lilí Díaz, Susana Vighi, Lucía Cardinal, Silvio Tatti, Nicasio Cúneo, Gonzalo Prat Gay, Gabriela Camporeale, Luis Alberto Palaoro, Argentophilic nucleolus organizer region as a proliferation marker in cervical intraepithelial neoplasia grade 1 of the uterine cervix. Journal of Obstetrics and Gynaecology Research. ,vol. 40, pp. 1717- 1724 ,(2014) , 10.1111/JOG.12380
王新, 张庆彬, 程成, 孔祥贵, NaYF4∶Yb3+, Er3+上转换发光纳米晶在三元混合溶剂体系中的配体交换修饰及发光性质 高等学校化学学报. ,vol. 35, pp. 224- 229 ,(2013) , 10.7503/CJCU20130779
Luis A Gómez, Glauco S Maciel, Cid B de Araújo, Amitava Patra, None, Upconversion in Er3+-doped ZrO2 nanocrystals pumped at 1.426μm Journal of Applied Physics. ,vol. 103, pp. 053507- ,(2008) , 10.1063/1.2844468
T. López-Luke, E. De la Rosa, P. Salas, C. Angeles-Chavez, L. A. Díaz-Torres, S. Bribiesca, Enhancing the Up-Conversion Emission of ZrO2:Er3+ Nanocrystals Prepared by a Micelle Process Journal of Physical Chemistry C. ,vol. 111, pp. 17110- 17117 ,(2007) , 10.1021/JP072957V
Andrei A. Bunaciu, Elena Bacalum, Hassan Y. Aboul-Enein, Gabriela Elena Udristioiu, Şerban Fleschin, FT-IR Spectrophotometric Analysis of Ascorbic Acid and Biotin and their Pharmaceutical Formulations Analytical Letters. ,vol. 42, pp. 1321- 1327 ,(2009) , 10.1080/00032710902954490
Tobias J. Brunner, Peter Wick, Pius Manser, Philipp Spohn, Robert N. Grass, Ludwig K. Limbach, Arie Bruinink, Wendelin J. Stark, In Vitro Cytotoxicity of Oxide Nanoparticles: Comparison to Asbestos, Silica, and the Effect of Particle Solubility† Environmental Science & Technology. ,vol. 40, pp. 4374- 4381 ,(2006) , 10.1021/ES052069I
Feng Gu, Shu Fen Wang, Meng Kai Lü, Guang Jun Zhou, Su Wen Liu, Dong Xu, Duo Rong Yuan, Effect of Dy3+ doping and calcination on the luminescence of ZrO2 nanoparticles Chemical Physics Letters. ,vol. 380, pp. 185- 189 ,(2003) , 10.1016/J.CPLETT.2003.09.011
Guanying Chen, Gabriel Somesfalean, Yan Liu, Zhiguo Zhang, Qiu Sun, Fuping Wang, Upconversion mechanism for two-color emission in rare-earth-ion-doped Zr O 2 nanocrystals Physical Review B. ,vol. 75, pp. 195204- ,(2007) , 10.1103/PHYSREVB.75.195204
V H Romero, E De la Rosa, T López-Luke, P Salas, C Angeles-Chavez, Brilliant blue, green and orange?red emission band on Tm3+-, Tb3+- and Eu3+-doped ZrO2 nanocrystals Journal of Physics D. ,vol. 43, pp. 465105- ,(2010) , 10.1088/0022-3727/43/46/465105
Bor-Chyuan Hwang, Shibin Jiang, Tao Luo, Jason Watson, Gino Sorbello, Nasser Peyghambarian, Cooperative upconversion and energy transfer of new high Er 3+ - and Yb 3+ –Er 3+ -doped phosphate glasses Journal of The Optical Society of America B-optical Physics. ,vol. 17, pp. 833- 839 ,(2000) , 10.1364/JOSAB.17.000833