Nucleotide sequence and genetic characterization of the novel IncQ-like plasmid pIE1107.
作者： Erhard Tietze
关键词: Nucleotide 、 Replication Initiation 、 Genetics 、 Replication (statistics) 、 Biology 、 Origin of replication 、 Plasmid 、 Nucleic acid sequence 、 Replicon 、 DNA
摘要: Abstract The analysis of the complete nucleotide sequence small resistance plasmid pIE1107 revealed a close similarity to well-known IncQ plasmids. Highly conserved replication proteins and nearly identical origins (oriV) suggest equivalent functions in related systems. However, contains two copies IncQ-oriV-like DNA which are slightly different regarding iterons. Upon deletion silent copy resulting is fully compatible with plasmids, indicating that there no mutual communication between control respective replicons. Experiments clonedoriV strongly initiation protein has specialized into distinct target-iterons its ownoriV differs only by few nucleotides from theoriV Implications apparent highly specific protein–DNA recognition incompatibility properties for evolution family compatible, IncQ-like plasmids discussed.
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