Dinitrogenase-Driven Photobiological Hydrogen Production Combats Oxidative Stress in Cyanothece sp. Strain ATCC 51142.

摘要: ABSTRACT Photobiologically synthesized hydrogen (H 2 ) gas is carbon neutral to produce and clean combust, making it an ideal biofuel. Cyanothece sp. strain ATCC 51142 a cyanobacterium capable of performing simultaneous oxygenic photosynthesis H production, highly perplexing phenomenon because evolving enzymes are O sensitive. We employed system-level in vivo chemoproteomic profiling approach explore the cellular dynamics protein thiol redox how mediates function dinitrogenase NifHDK, enzyme complex aerobic hydrogenase activity. found that NifHDK responds intracellular conditions may act as emergency electron valve prevent harmful reactive oxygen species formation concert with other cell strategies for maintaining homeostasis. These results provide new insight into useful advancing photolytic bioenergy technology reveal understanding biological NifHDK. IMPORTANCE Here, we demonstrate high levels synthesis can be induced protection mechanism against oxidative stress via 51142. This previously unknown feature cyanobacterial dinitrogenase, anticipate represent strategy exploit cyanobacteria efficient scalable production. utilized capture situ reductant partitioning within cell, revealing proteins thiols involved sensing signaling. Additionally, this method widely applicable across systems achieve greater cells navigate their environment chemistry alter metabolism