Selection of salt-tolerant doubled haploids in rice anther culture
作者： Seung Yeob Lee
关键词: Salt (chemistry) 、 Biology 、 Botany 、 Stamen 、 Hybrid 、 Japonica 、 Callus 、 Agar plate 、 Plant physiology 、 Horticulture 、 Backcrossing
摘要: For the high frequency selection of salt-tolerant doubled haploids (DHs) using rice anther culture, efficiency culture was investigated with different genotype, media condition and NaCl concentrations. The six F1 hybrids obtained by backcross or three-way cross between indica japonica differed in salt tolerance. efficiencies callus induction plant regeneration decreased concentration tolerance donor variety, those japonicas were higher than indicas. percentages Gyehwa 5 (japonica, tolerant) IR61633-B-2-2-1 sensitive) 21.1 13.5% on agar medium containing 0.3% NaCl, respectively. derived from floating liquid less that solid medium. In four hybrids, frequencies DHs 21.4 8.9% control, could be selected (0.3% NaCl) combinations crossed as third parent.
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