Cartilage Oligomeric Matrix Protein promotes epithelial-mesenchymal transition by interacting with Transgelin in Colorectal Cancer.

摘要: Background and Purpose: The role of the cartilage oligomeric matrix protein (COMP) in epithelial-mesenchymal transition (EMT) tumor progression has been studied, but its exact regulatory mechanism remains unknown. Methods: interaction between COMP actin-binding transgelin (TAGLN) was identified by prediction co-immunoprecipitation verified through stochastic optical reconstruction microscopy (STORM) duolink experiments. Western blot immunofluorescence analyses were conducted to detect changes EMT-related markers after overexpression knockdown. Molecular docking Biacore interface COMP/TAGLN revealed that Chrysin directly targeted COMP. promotion inhibition EMT detected cell migration, invasion, apoptosis, xenotransplantation nude mice. Results: interacts with TAGLN colorectal cancer regulate cytoskeletal remodeling promote malignant progression. is highly expressed positively correlated expression. knockdown can inhibit metastasis whereas promotes cancer. Through virtual screening interface, Chrysin, a flavonoid compound extracted from Oroxylum indicum, found have highest score complex. inhibited COMP, thereby preventing Conclusions: This study illustrated suggested may be potential therapeutic target. exhibits obvious antitumor effects. work provides preliminary therapy target or EMT.