Recent progress in liquid chromatography-based separation and label-free quantitative plant proteomics
作者： A. Matros , S. Kaspar , K. Witzel , H.-P. Mock
DOI: 10.1016/J.PHYTOCHEM.2010.11.009
关键词: Nucleotide sequencing 、 Label free 、 Quantitative proteomics 、 Separation technology 、 Database search engine 、 Proteome 、 Chemistry 、 Proteomics 、 Chromatography 、 Label-free quantification
摘要: Recent innovations in liquid chromatography-mass spectrometry (LC-MS)-based methods have facilitated quantitative and functional proteomic analyses of large numbers proteins derived from complex samples without any need for protein or peptide labelling. Regardless its great potential, the application these proteomics techniques to plant science started only recently. Here we present an overview label-free features their employment analysing plants. used by MS are summarized major challenges associated with LC-MS-based approaches, including sample preparation, separation, quantification kinetic studies, discussed. Database search algorithms specific aspects regarding identification non-sequenced organisms also addressed. So far, LC-MS has been establish cellular subcellular proteome maps, characterize plant-pathogen interactions stress defence reactions, profiling patterns during developmental processes. Improvements both, analytical platforms (separation technology bioinformatics/statistical analysis) high throughput nucleotide sequencing technologies will enhance power this method.
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