Comparison of nested PCR with immunofluorescent-antibody assay for detection of Ehrlichia canis infection in dogs treated with doxycycline.
作者： B Wen , Y Rikihisa , J M Mott , R Greene , H Y Kim
DOI: 10.1128/JCM.35.7.1852-1855.1997
关键词: Ehrlichia muris 、 Antibacterial agent 、 Ehrlichiosis (canine) 、 Canis 、 Virology 、 Ehrlichia chaffeensis 、 Ehrlichia canis 、 Biology 、 Nested polymerase chain reaction 、 Ehrlichia
摘要: A partial 16S rRNA gene was amplified in Ehrlichia canis-infected cells by nested PCR. The assay specific and did not amplify the closely related chaffeensis, muris, Neorickettsia helminthoeca, SF agent genes. as sensitive Southern hybridization, detecting little 0.2 pg of E. canis DNA. By this method, all blood samples from four dogs experimentally infected with were positive early day 4 postinoculation, which before or at time seroconversion. One hundred five Arizona Texas (areas endemicity) 30 Ohio (area nonendemicity) examined PCR immunofluorescent-antibody (IFA) test. Approximately 84% had been treated doxycycline submission specimens. Among specimens, 46 (44%) 80 IFA (76%). Forty-three IFA-positive (54%) positive, 22 25 IFA-negative (88%) negative None specimens but 5 (17%). Our results indicate that is highly for detection may be more useful assessing clearance organisms after antibiotic therapy than IFA, especially areas endemic.
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