Observation of inverted cubic phase in hydrated dioleoylphosphatidylethanolamine membranes.
作者： E Shyamsunder , Sol M Gruner , MW Tate , DC Turner , PTC So
DOI: 10.1021/BI00407A014
关键词: Lattice (order) 、 Metastability 、 Crystallography 、 Phase transition 、 Diffraction 、 Nuclear magnetic resonance 、 Lamellar structure 、 Hexagonal phase 、 X-ray crystallography 、 Chemistry 、 Small-angle X-ray scattering
摘要: We report the observation of an inverted cubic phase in aqueous dispersions 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) by small-angle X-ray diffraction. DOPE is a paradigm study nonlamellar phases biological systems: it exhibits well-known transition from lamellar (L alpha) to hexagonal (HII) as temperature raised. The observed occur rapidly when dispersion heated 2 degrees C, where L alpha stable, 15 HII stable. on induction crystallographically well-defined lattice that slowly formed lipid cycled between -5 and C hundreds times. Once formed, stable at 4 for several weeks same remarkable metastability characterizes other lipid-water systems. diffraction indicates most consistent with either Pn3m or Pn3 space group. Tests purity after indicate least 98% pure. can be destroyed system reset cycling specimen times -30 C. kinetics formation are dependent thermal history sample, overall water concentration, extreme temperatures cycle.(ABSTRACT TRUNCATED AT 250 WORDS)
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sci-hub.se 参考文章(19)
Gregory L. Kirk, Sol M. Gruner, D. L. Stein, A thermodynamic model of the lamellar to inverse hexagonal phase transition of lipid membrane-water systems Biochemistry. ,vol. 23, pp. 1093- 1102 ,(1984) , 10.1021/BI00301A009
K. LARSSON, Two cubic phases in monoolein–water system Nature. ,vol. 304, pp. 664- 664 ,(1983) , 10.1038/304664C0
Sol M. Gruner, James R. Milch, George T. Reynolds, Slow-scan silicon-intensified target-TV x-ray detector for quantitative recording of weak x-ray images Review of Scientific Instruments. ,vol. 53, pp. 1770- 1778 ,(1982) , 10.1063/1.1136895
D.P. Siegel, Inverted micellar intermediates and the transitions between lamellar, cubic, and inverted hexagonal amphiphile phases. III. Isotropic and inverted cubic state formation via intermediates in transitions between Lα and HII phases Chemistry and Physics of Lipids. ,vol. 42, pp. 279- 301 ,(1986) , 10.1016/0009-3084(86)90087-3
Annette Gulik, Vittorio Luzzati, Mario De Rosa, Agata Gambacorta, Structure and polymorphism of bipolar isopranyl ether lipids from archaebacteria Journal of Molecular Biology. ,vol. 182, pp. 131- 149 ,(1985) , 10.1016/0022-2836(85)90032-4
Sol M. Gruner, V. Adrian Parsegian, R. Peter Rand, Directly measured deformation energy of phospholipid HII hexagonal phases Faraday Discussions of The Chemical Society. ,vol. 81, pp. 29- 37 ,(1986) , 10.1039/DC9868100029
W Helfrich, Elastic Properties of Lipid Bilayers: Theory and Possible Experiments Zeitschrift für Naturforschung C. ,vol. 28, pp. 693- 703 ,(1973) , 10.1515/ZNC-1973-11-1209
James A. Ibers, Lonsdale, Kathleen, Dame, John S. Kasper, Gerard D. Rieck, Walter C. Hamilton, Norman Fordyce McKerron Henry, Caroline H. MacGillavry, International tables for X-ray crystallography UT Back-in-Print Service. ,(1962)
John M. Seddon, Gregor Cevc, R. D. Kaye, Derek Marsh, X-ray diffraction study of the polymorphism of hydrated diacyl- and dialkylphosphatidylethanolamines. Biochemistry. ,vol. 23, pp. 2634- 2644 ,(1984) , 10.1021/BI00307A015
William Longley, Thomas J. McIntosh, A bicontinuous tetrahedral structure in a liquid-crystalline lipid Nature. ,vol. 303, pp. 612- 614 ,(1983) , 10.1038/303612A0