作者: Bianca K. Wetzl , Sergiy M. Yarmoluk , Douglas B. Craig , Otto S. Wolfbeis
关键词: Electrophoresis 、 Fluorescence 、 Bradford protein assay 、 Biochemistry 、 Proteomics 、 Chemistry 、 Staining 、 A protein 、 Covalent bond
摘要: Glowing marks: A new class of protein stains, the pyrylium dyes, undergo a strong color change (typically from blue to red, see picture) on covalently binding proteins. While free stains are almost nonfluorescent, protein-conjugated forms highly fluorescent. The dyes do not alter charge protein, and thus its electrophoretic properties. also can be used in quantitative assays.