Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays).
作者： Max Schubert , Marcel Houdelet , Karl-Heinz Kogel , Rainer Fischer , Stefan Schillberg
DOI: 10.1007/S11248-015-9888-2
关键词: Expression cassette 、 Biology 、 Aspergillus parasiticus 、 Microbiology 、 Botany 、 Genetically modified maize 、 Aspergillus 、 Antimicrobial peptides 、 Heterologous expression 、 Genetically modified crops 、 Aspergillus flavus
摘要: Aflatoxin-producing fungi can contaminate plants and plant-derived products with carcinogenic secondary metabolites that present a risk to human animal health. In this study, we investigated the effect of antimicrobial peptides on major aflatoxigenic Aspergillus flavus A. parasiticus. vitro assays different chemically-synthesized demonstrated broad-spectrum peptide thanatin from spined soldier bug (Podisus maculiventris) had greatest potential eliminate fungi. The minimal inhibitory concentrations against A. parasiticus were 3.13 12.5 µM, respectively. A cDNA was subsequently cloned in plant expression vector under control ubiquitin-1 promoter allowing recombinant be directed apoplast transgenic maize plants. Successful integration cassette confirmed by PCR semi-quantitative RT-PCR kernels. Infection kernels T1 showed up three-fold greater resistance spp. infections compared non-transgenic We for first time heterologous inhibits growth offering solution protect crops aflatoxin-producing resulting aflatoxin contamination field storage conditions.
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