Liver microsomal levels of cytochrome P450IA1 as biomarker for exposure and bioavailability of soil-bound polycyclic aromatic hydrocarbons

摘要: The bioavailability of soil-bound polycyclic aromatic hydrocarbons (PAHs) for mammalian species was studied with rats fed a diet containing contaminated soil preparations. extent cytochrome P450IA1 (CYP1A1) induction in the liver correlated amount 5- and 6-ring PAHs samples but not total PAH content. Other cytochromes P450 were much less affected by soil-contaminants. highest CYP1A1 obtained sample 274 mg PAH/kg soil, resulting nearly 360-fold increase ethoxyresorufin deethylase (EROD) activity. In semilogarithmic plot, linear correlation found between concentration microsomal As model action intestinal fluids, extracted bile acid solution. these experiments, selectivity solubilization individual parallels that toluene extraction, although yield is lower than latter varies sample. microorganisms, mammals, shown to be considerably reduced presence high organic carbon (TOC) values samples. This may have implications decontamination strategies, diminishing effectiveness biological cases TOC values. data suggest parameter useful risk assessments soils species.