Human papilloma virus and p53 in head and neck cancer: clinical correlates and survival.

摘要: Recent studies have shown that p53 mutations are frequently found in cancer of the head and neck, whereas others indicated human papilloma virus (HPV) infection may be involved. Thus far, no examined both HPV same patient population correlated results with clinical characteristics outcome. The purpose this study was to examine any interrelationship between patients squamous cell carcinoma (SCC) neck. We also planned correlate experimental findings characteristics, known risk factors, treatment outcome determine whether prognostic factors could detected. Archival material from 66 SCC neck were selected for based on availability tissue primary tumors prior treatment. A data base constructed containing all parameters at time diagnosis factors. Genomic DNA isolated amplified using PCR, followed by SSCP analysis direct genomic sequencing variants detect mutations. Two independent methods used detection: (a) PCR amplification primers homologous E6 region 16, 18, 33, RFLP analysis; (b) L1 consensus primers, triple restriction enzyme digestion. entered into statistical analysis. Twenty-four percent mutations, 18% positive infection. Only one both. Tonsilar strongly (P = 0.0001) inversely 0.03). only parameter associated mutation a trend toward heavier smoking history. subset tonsilar revealed inverse correlations 0. 015) alcohol use 0.05). Also, white tonsil more likely 0.015). No significant relationships detected either or entire population. stage IV disease overall survival. This is largest date Our suggest involved development these cancers without traditional HPV-related prevalent race.