MiR-154 Functions as a Tumor Suppressor in Glioblastoma by Targeting Wnt5a
作者： Dongsheng Zhao , Rencong Wang , Junkang Fang , Xituan Ji , Juan Li
DOI: 10.1007/S12035-016-9867-5
关键词: microRNA 、 WNT5A 、 Molecular biology 、 Cancer research 、 Gene expression profiling 、 Cell migration 、 Biology 、 Apoptosis 、 U87 、 Epithelial–mesenchymal transition 、 Downregulation and upregulation
摘要: Recently, deregulated microRNA (miRNA) expression contributes to the development and progression of human glioblastoma. The aim present study was evaluate level miR-154 Wnt5a in glioblastoma tissues cells. We further investigated molecular mechanisms cell lines. In study, we found that downregulated U87, U251, A172 cells (all p < 0.001). By contrast, upregulated. Furthermore, overexpression suppressed migration invasion U87 U251 Mechanically, inhibited epithelial-to-mesenchymal transition (EMT) Importantly, identified 3' untranslated region (3'-UTR) a direct target miR-154. Luciferase reporter assays confirmed binding 3'-UTR regions At same time, overexpressed also reversed EMT by conclusion, this suggested high migration, invasion, through targeting Wnt5a, which may be recommended as therapeutic for
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