Isolation and characterization of antagonistic bacteria with the potential for biocontrol of soil-borne wheat diseases

摘要: Bacillus amyloliquefaciens subsp. plantarum XH-9 is a plant-beneficial rhizobacterium that shows good antagonistic potential against phytopathogens by releasing diffusible and volatile antibiotics, secreting hydrolytic enzymes. Furthermore, the strain possesses important plant growth-promoting characteristics, including nitrogen fixation (7.92 ± 1.05 mg/g), phosphate solubilization (58.67 4.20 µg/L), potassium (10.07 1.26 µg/mL), presence of siderophores (4.92 0.46 indole-3-acetic acid (IAA) (7.76 0.51 µg/mL) 1-aminocyclopropane-1-carboxylic deaminase (ACC-deaminase) (4.67 1.21 nmol/[mg·h]). Moreover, showed capacities for wheat, corn, chili root colonization, which are critical prerequisites controlling soil-borne diseases as bio-control agent. Real-time quantitative polymerase chain reaction experiments amount Fusarium oxysporum DNA associated with after treatment significantly decreased compared control group. Accordingly, wheat plants inoculated significant increases in shoot heights (14.20%), lengths (32.25%), dry biomass levels (11.93%), fresh (16.28%) relative to un-inoculated plants. The results obtained this study suggest has plant-growth promoter biocontrol agent when applied local arable land prevent damage caused F. other phytopathogens.