Electrochemical sandwich-type thrombin aptasensor based on dual signal amplification strategy of silver nanowires and hollow Au-CeO2.

摘要: Abstract Signal amplification is crucial in electrochemical biosensor to obtain low detection limits. In this work, a highly sensitive sandwich-type thrombin aptasensor constructed, based on dual signal of uniform silver nanowires (AgNWs) and hollow Au–CeO2 nanocomposites. AgNWs are decorated the ITO surface immobilize amino functionalized capture apemeter 1 (Apt1). And Au nanoparticles (AuNPs) grown directly CeO2 microstructure used sulfydryl reporter 2 (Apt2). Thus, apatasensor has been successfully designed, due specific recognition between two kinds aptamers. One amplifications from good conductivity AgNWs. Moreover, together with exhibit catalytic performance for reduction H2O2, further resulting significant amplification. Because closely related concentration, differential pulse voltammetry specifically detect thrombin. Under optimized conditions, proposed method linear response ranged 0.5 pM 30 nM limit 0.25 (S/N = 3) The displays selectivity, reproducibility stability, providing platform ultrasensitive