Toxoplasma gondii in Ireland: seroprevalence and novel molecular detection method in sheep, pigs, deer and chickens.

摘要: Toxoplasma gondii is among the most studied parasites worldwide but there not much information about it published in Ireland. The objectives of this study were to determine seroprevalence T. sheep, pigs, deer and chickens molecular detection DNA muscle tissue. Serum samples collected from these species at time slaughter Irish abattoirs during 2007 tested for anti-T. antibodies using a commercial semi-quantitative latex agglutination test. Antibodies (titre ≥1 : 64) found 36% (105/292) 4.7% (15/317) pigs 6.6% (23/348) deer. In chickens, 18% (65/364) had antibody titres, ranging between 1 5 1024. Significant (P ≤ 0.05) age-related differences adult sheep (58.1%) (23.1%). gender was also with more females (43%) than males (22.4%) being positive. However, when adjusted age through logistic regression no longer significant. Seroprevalence evaluated on farm locations grouped NUTS level 3, prevalence too low draw any statistical conclusions. Using nested PCR, presence detected diaphragm 3.6% (3/83) 13.0% (3/23) pig 4.2% (3/71) Meat digestion liquids Trichinella spp. survey used first detect gondii. 50% (10/20) pooled samples. This depth animals Ireland novel method, liquid samples, PCR described.