A SHORT INTERFERING RNA MOLECULAR BEACON FOR THE ATTENUATION OF MYCOBACTERIAL INFECTION

摘要: The ability of the pathogen Mycobacterium Tuberculosis (MTB) to invade and survive within macrophages granulomas is attributed product Mammalian Cell Entry (MCE) operon whose gene, mce4A , encodes a cholesterol transporter that transports h ost lipids into bacterium allows bacte rium during chronic infection. Here, we proposed tested hypothesis siRNA molecular beacon can be used attenuate mycobacterial infec tion in macrophages. Mce4A gene was cloned expressed E. coli (E. coli-4A ) differentiated U937 cells were transduced wit piLenti-siRNA -GFP phage expressing for 24 h. This followed by infection either or M. smegmatis 3 incubation 0, 3, 6, 48 . lysed lysates plated on LB agar plates containing ampicillin (100 µg mL -1 7H11 media incubated at 37°C overnight. Our results showed treatment attenuated E.coli-4A 0 77, 59.6 99.7%, respectively. also show ed 9 4.8, 70.3, 98.9 93.4%, In conclus ion, successfully delivered stably which coli-4A)