Circulating osteoblast-lineage cells in humans.

摘要: Background Although current evidence suggests that only a minuscule number of osteoblast-lineage cells are present in peripheral blood, we hypothesized such circulate but their concentration has been vastly underestimated owing to the use assays required adherence plastic. We further reasoned these is elevated during times increased bone formation, as pubertal growth. Methods used flow cytometry with antibodies bone-specific proteins identify circulating 11 adolescent males and adult (mean [±SD] age, 14.5±0.7 vs. 37.7±7.6 years). Gene expression vitro vivo bone-forming were establish osteoblastic lineage sorted cells. Results Cells positive for osteocalcin alkaline phosphatase detected blood subjects (1 2 percent mononuclear cells). There more than five many ...