The microbiome is essential for normal gut intrinsic primary afferent neuron excitability in the mouse

摘要: Background  The role of intestinal microbiota in the development and function host physiology is high interest, especially with respect to nervous system. While strong evidence has accrued that bacteria alter system function, mechanisms by which this occurs have remained elusive. For reason, we carried out experiments examining electrophysiological properties neurons myenteric plexus enteric (ENS) germ-free (GF) mice compared specific pathogen-free (SPF) control adult been conventionalized (CONV-GF) bacteria. Methods  Segments jejunum from 8 12 week old GF, SPF, CONV-GF were dissected expose plexus. Intracellular recordings current-clamp mode made impaling cells sharp microelectrodes. Action potential (AP) shapes, firing thresholds, number APs fired at 2× threshold, passive membrane characteristics measured. Key Results  In GF mice, excitability was decreased afterhyperpolarization (AH) as measured a lower resting generated threshold. post AP slow (sAHP) prolonged for SPF animals. Passive also altered decrease input resistance. Conclusions & Inferences  Here, report novel finding commensal are necessary normal gut sensory thus provide mechanism transfer information between