Binding of cowpea chlorotic mottle virus to cowpea protoplasts and relation of binding to virus entry and infection.

摘要: Abstract Cowpea chlorotic mottle virus (CCMV) and cowpea protoplasts were used to study initial interactions between protoplast. Protoplasts incubated under varying conditions of temperature, pH, ionic strength, the presence added compounds. Both amount 35S-labeled bound percentage infected cells determined. At 0 25° associated with increased added. With inoculum 25 × 106 particles per protoplast, 4 103 14 protoplast at 25°, respectively. In polyethylene glycol, 85 both temperatures ca. 50% became infected. No infection occurred in absence PEG. Variation pH or strength PEG caused little no change binding infection. PEG, increase resulted lower binding, but infectivity was not affected. Increasing however, infectivity. The unlabeled CCMV, tobacco mosaic coat protein, bovine serum albumin, polycations during inoculation decreased CCMV. contrast, CCMV which has a positively charged N-terminal arm, binding. effects similar, although larger amounts bound. reduced by all additives 5–25%. Addition ammonium chloride, inhibits endocytotic uptake animal cells, as well culture media, did reduce These data do support specific receptor-mediated favor nonspecific mechanism entry, possibly through membrane lesions. Observations electron microscope latter mechanism.