Tunable Aqueous Polymer Phase Impregnated Resins (TAPPIR®): Investigation of the impregnation stability

摘要: Abstract Aqueous Two-Phase Extraction (ATPE) is known to be a gentle separation technique for biochemical products due the high water content of aqueous two-phase system (ATPS) used. However, phases' physicochemical properties like low interfacial tension, viscosities and small density differences can lead long phase times. This major drawback renders classical ATPE uneconomical industrial application as additional equipment energy needed promote faster separation. Our approach enhance Tunable Polymer Phase Impregnated Resins (TAPPIR®)-Technology, where one an ATPS immobilized inside porous solids, which are then dispersed in other phase. A stable impregnation during TAPPIR® decides upon competitiveness protein recovery techniques. Therefore stability dependent on process (temperature pH value), particles parameters was investigated. As many need measured, making determination predicting parameter difficult, three-phase contact angle proposed describe interaction with solid. It found, that impregnability phases could predicted angle. Thus, selection impregnated design novel tasks simplified.