Transgenic rice lines constitutively co-expressing tlp-D34 and chi11 display enhancement of sheath blight resistance
作者： J. M. Shah , R. Singh , K. Veluthambi
DOI: 10.1007/S10535-012-0291-Z
关键词: Northern blot 、 Southern blot 、 Molecular biology 、 Transformation (genetics) 、 Western blot 、 Genetics 、 Biology 、 Transgene 、 Agrobacterium tumefaciens 、 Genetically modified rice 、 Agrobacterium
摘要: Transgenic rice (Oryza sativa L. subsp. indica cv. White Ponni) constitutively expressing the thaumatin-like protein gene (tlp-D34, PR-5) individually or in combination with chitinase (chi11, PR-3) was generated using an Agrobacterium vir helper strain multiple copies of pTiBo542 virB and virG. Transformation tlp-D34 alone + chi11 genes yielded five seven single-copy transgenic lines, respectively. Southern blot analysis two probes, one flanking right T-DNA border second left border, confirmed that all plants harboured single complete copies. Homozygous lines were first identified T1 generation by subsequently segregation T2 plants. Accumulation transcripts encoded transgenes T0 homozygous Northern analysis. The harbouring showed 2.8- to 4.2-fold higher activity. Western revealed accumulation respective Upon infection Rhizoctonia solani, disease index reduced from 100 % control 65 a T3 line T4 alone. In CT22 co-expressing genes, 39 %.
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