Amplification of 16S rRNA genes from culturable and nonculturable mollicutes

摘要: Abstract Five polymerase chain reaction (PCR) primer pairs were synthesized on the basis of aligned 16S-like rRNA sequences eukaryotes or 16S eubacteria, Mollicutes, and intracellular organelles. These PCR had high sequence homology to conserved genes various culturable nonculturable but less eukaryotic nuclear Full-length partial-length evolutionarily variable regions successfully amplified when DNA preparations from Mollicutes such as Mycoplasma flocculare three Spiroplasma strains associated with plant diseases used templates. Amplifications not detected Escherichia coli genomic healthy plants under stringency annealing conditions in thermocycling. The results suggest possibility that can be for detection a phylogenetic study using crude appropriately controlled thermocycling conditions.