Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice

摘要: Carbonic anhydrases are a family of enzymes that catalyze the reversible condensation water and carbon dioxide to carbonic acid, which spontaneously dissociates bicarbonate. anhydrase III (Car3) is nutritionally regulated at both mRNA protein level. It highly enriched in tissues synthesize and/or store fat: liver, white adipose tissue, brown skeletal muscle. Previous characterization Car3 knockout mice focused on fed standard diets, not high-fat diets significantly alter express Car3. We observed lower levels diet treated with niclosamide, drug published improve fatty liver symptoms mice. However, it unknown if simply biomarker reflecting lipid accumulation or whether has functional role regulating metabolism. our vitro studies toward metabolic pathways require To further determine metabolism, we measured de novo acid synthesis radiolabeled experiments examined biomarkers wild type diet. Specifically, analyzed body weight, composition, rate, insulin resistance, serum tissue triglycerides. Our results indicate required for lipogenesis, do have significant differences responses various