The expression of UDP-glucuronosyltransferases of the UGT1 family in human liver and kidney and in response to drugs.
作者： Lesley Sutherland , Thomas Ebner , Brian Burchell
DOI: 10.1016/0006-2952(93)90064-4
关键词: Gene isoform 、 Isozyme 、 Glucuronidation 、 Phenobarbital 、 Chemistry 、 Insulin 、 Endocrinology 、 Kidney 、 Gene expression 、 Bilirubin 、 Internal medicine
摘要: The expression of human UDP-glucuronosyltransferase (UGT) 1 gene family in the liver and kidney was examined using specific enzyme activity, antibodies DNA probes for each four members. Phenol UGT HP1 expressed at a similar, relatively low, abundance whereas phenol HP4 more highly kidney. Bilirubin UGTs (HP2 HP3) were not detectable HP3 major isoform liver. activities towards certain substrates correlated well with respective mRNA levels tissues. induced 2-3-fold livers from patients treated phenytoin phenobarbital. Storage University Wisconsin solution which contains dexamethasone insulin caused large accumulation all mRNAs, but these quantitatively translated into activities. implications results are discussed.
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sci-hub.se 参考文章(25)
J Fevery, M Van de Vijver, R Michiels, K P M Heirwegh, Comparison in different species of biliary bilirubin-IX alpha conjugates with the activities of hepatic and renal bilirubin-IX alpha-uridine diphosphate glycosyltransferases. Biochemical Journal. ,vol. 164, pp. 737- 746 ,(1977) , 10.1042/BJ1640737
R Wooster, L Sutherland, T Ebner, D Clarke, O Da Cruz e Silva, B Burchell, Cloning and stable expression of a new member of the human liver phenol/bilirubin: UDP-glucuronosyltransferase cDNA family Biochemical Journal. ,vol. 278, pp. 465- 469 ,(1991) , 10.1042/BJ2780465
J K Ritter, Y Y Sheen, I S Owens, Cloning and expression of human liver UDP-glucuronosyltransferase in COS-1 cells. 3,4-catechol estrogens and estriol as primary substrates. Journal of Biological Chemistry. ,vol. 265, pp. 7900- 7906 ,(1990) , 10.1016/S0021-9258(19)39016-7
J.K. Ritter, F Chen, Y.Y. Sheen, H.M. Tran, S Kimura, M.T. Yeatman, I.S. Owens, A novel complex locus UGT1 encodes human bilirubin, phenol, and other UDP-glucuronosyltransferase isozymes with identical carboxyl termini. Journal of Biological Chemistry. ,vol. 267, pp. 3257- 3261 ,(1992) , 10.1016/S0021-9258(19)50724-4
J S He, A J Fulco, A barbiturate-regulated protein binding to a common sequence in the cytochrome P450 genes of rodents and bacteria. Journal of Biological Chemistry. ,vol. 266, pp. 7864- 7869 ,(1991) , 10.1016/S0021-9258(20)89529-5
K W Bock, C von Bahr, W Lilienblum, Studies of UDP-glucuronyltransferase activities in human liver microsomes. Drug Metabolism and Disposition. ,vol. 12, pp. 93- 97 ,(1984)
B Burchell, T Ebner, Substrate specificities of two stably expressed human liver UDP-glucuronosyltransferases of the UGT1 gene family. Drug Metabolism and Disposition. ,vol. 21, pp. 50- 55 ,(1993)
S Fournel-Gigleux, MR Jackson, R Wooster, B Burchell, None, Expression of a human liver cDNA encoding a UDP-glucuronosyltransferase catalysing the glucuronidation of hyodeoxycholic acid in cell culture. FEBS Letters. ,vol. 243, pp. 119- 122 ,(1989) , 10.1016/0014-5793(89)80111-5
Genji Otani, Mahmud M. Abou-El-Makarem, Karl Walter Bock, UDP-glucuronyltransferase in perfused rat liver and in microsomes - III. Effects of galactosamine and carbon tetrachloride on the glucuronidation of 1-naphthol and bilirubin. Biochemical Pharmacology. ,vol. 25, pp. 1293- 1297 ,(1976) , 10.1016/0006-2952(76)90092-7
Birgit L. Coffman, Thomas R. Tephly, Yacoub M. Irshaid, Mitchell D. Green, Christine Smith, Michael R. Jackson, Richard Wooster, Brian Burchell, Characterization and primary sequence of a human hepatic microsomal estriol UDPglucuronosyltransferase Archives of Biochemistry and Biophysics. ,vol. 281, pp. 170- 175 ,(1990) , 10.1016/0003-9861(90)90428-2