V-ATPase subunit B plays essential roles in the molting process of Chinese mitten crab, Eriocheir sinensis.

摘要: Vacuolar ATPase (V-ATPase) is a proton pump driven by ATP hydrolysis, and it plays important roles in numerous biological processes, such as protein degradation synthesis, cell growth, autophagy. The V-ATPase subunit B (VATB) conservative regulatory required for hydrolysis during pumping. VATB of E. sinensis (EsVATB), which includes an open reading frame (ORF) length 1467 bp encoding 489 amino acids, was cloned to unveil the function molting process crustaceans. Spatial temporal expression profiles showed that EsVATB highly expressed posterior gill accompanied with highest osmotic pressure premolt (PrM) stage. Meanwhile, level identified hepatopancreas heart postmolt stage epidermis intermolt stage, indicating may perform diverse functions different tissues process. individual crabs interference group high mortality rate (74%) low (26%) failed form new epicuticle PrM significant difference between control groups. Our results indicate indispensable functional gene participate osmoregulation help formation sinensis.