The HSD-hCG Vaccine Prevents Pregnancy in Women: Feasibility Study of a Reversible Safe Contraceptive Vaccine
作者： G.P. Talwar , Om Singh , S.K. Gupta , S.E. Hasnain , R. Pal
DOI: 10.1111/J.1600-0897.1997.TB00207.X
关键词: Pregnancy 、 Medicine 、 Ovulation 、 Immunogenicity 、 Gonadotropin 、 Endocrinology 、 Internal medicine 、 Epitope 、 Toxoid 、 Antibody 、 Tetanus 、 Obstetrics and gynaecology 、 Immunology 、 Immunology and Allergy 、 Reproductive medicine
摘要: Both a Phase I multicenter safety trial in 47 women with elective tubal ligation and II efficacy studies 148 IUD users from India confirmed the feasibility of an HSD-human chorionic gonadotropin (hCG) vaccine for reversible contraception. HSD is heterospecies dimer beta-subunit hCG associated noncovalently alpha-subunit ovine gonadotropin; was selected as target immuno-interception to avert interference pituitary-ovarian axis. In trials all produced anti-hCG antibodies no impairment ovulation or menstrual regularity. were again elicited but only 119 (80%) made above 50 ng/ml bioneutralizing capacity. When titers exceeded this level IUDs removed. Only 1 pregnancy at levels exceeding occurred over 1224 cycles observation. 65 protected 6-24 continuous exposure additional contraceptive method use. As fell below 35 pregnancies occurred. The antibody response humans characterized predominantly epitope recognized by monoclonals 206 P3W80. had low reactivity carboxyl terminal peptide 38-57 region peptide. effectiveness HSD-hCG could be further optimized replacement tetanus diphtheria toxoid carriers T non B determinants development recombinant expressing beta-hCG along membrane anchored carrier.
sci-hub.se 参考文章(23)
Noel R. Rose, C. Lynne Burek, Julian P. Smith, Safety Evaluation of HCG Vaccine in Primates: Autoantibody Production Contraception Research for Today and the Nineties. pp. 231- 239 ,(1988) , 10.1007/978-1-4612-3746-4_21
J Srinivasan, O Singh, S Chakrabarti, G P Talwar, Targeting vaccinia virus-expressed secretory beta subunit of human chorionic gonadotropin to the cell surface induces antibodies. Infection and Immunity. ,vol. 63, pp. 4907- 4911 ,(1995) , 10.1128/IAI.63.12.4907-4911.1995
M. Salahuddin, C. Das, S. Ramakrishnan, S. Kumar, V. Hingorani, G. P. Talwar, N. C. Sharma, S. K. Dubey, Isoimmunization against human chorionic gonadotropin with conjugates of processed beta-subunit of the hormone and tetanus toxoid. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 73, pp. 218- 222 ,(1976) , 10.1073/PNAS.73.1.218
Sekhar Chakrabarti, J. Srinivasan, Lavanya Lall, L.V. Rao, Gursaran P. Talwar, Expression of biologically active human chorionic gonadotropin and its subunits by recombinant vaccinia virus. Gene. ,vol. 77, pp. 87- 93 ,(1989) , 10.1016/0378-1119(89)90362-4
U. S. Deshmukh, G. P. Talwar, S. K. Gupta, Antibody response against three epitopic domains on human chorionic gonadotropin (hCG) in women and rodents immunized with a ?hCG-based immunocontraceptive vaccine Journal of Clinical Immunology. ,vol. 14, pp. 162- 168 ,(1994) , 10.1007/BF01533365
MalcolmW. Greaves, Rod Dawber, ZINC IN PSORIASIS The Lancet. ,vol. 295, pp. 1295- 1295 ,(1970) , 10.1016/S0140-6736(70)91776-9
W.R. Jones, S.J. Judd, R.M.Y. Ing, J. Powell, J. Bradley, E.H. Denholm, U.W. Mueller, P.D. Griffin, V.C. Stevens, PHASE I CLINICAL TRIAL OF A WORLD HEALTH ORGANISATION BIRTH CONTROL VACCINE The Lancet. ,vol. 331, pp. 1295- 1298 ,(1988) , 10.1016/S0140-6736(88)92117-4
VERNON C. STEVENS, B. CINADER, JOHN E. POWELL, ARTHUR C. LEE, S. W. KOH, Preparation and Formulation of a Human Chorionic Gonadotropin Antifertility Vaccine: Selection of Adjuvant and Vehicle* American Journal of Reproductive Immunology. ,vol. 1, pp. 315- 321 ,(1981) , 10.1111/J.1600-0897.1981.TB00064.X
Asok Mukhopadhyay, S.N. Mukhopadhyay, G.P. Talwar, Physiological factors of growth and susceptibility to virus regulating Vero cells for optimum yield of vaccinia and cloned gene product (β-hCG) Journal of Biotechnology. ,vol. 36, pp. 177- 182 ,(1994) , 10.1016/0168-1656(94)90053-1
Padma Sridhar, Amulya K Panda, Rahul Pal, Gursaran P Talwar, Seyed E Hasnain, None, Temporal nature of the promoter and not relative strength determines the expression of an extensively processed protein in a baculovirus system FEBS Letters. ,vol. 315, pp. 282- 286 ,(1993) , 10.1016/0014-5793(93)81179-4