New volumetric CNT-doped gelatin–cellulose scaffolds for skeletal muscle tissue engineering

摘要: Currently, the fabrication of scaffolds for engineered skeletal muscle tissues is unable to reach millimeter size. The main drawbacks are poor nutrient diffusion, lack an internal structure align precursor cells, and mechanical electric properties. Herein, we present a combination gelatin-carboxymethyl cellulose materials polymerised by cryogelation process that allowed us scaffold up size solve problems related large tissue constructs. (1) By incorporating carbon nanotubes (CNT), can improve electrical properties scaffold, thereby enhancing maturation when applying pulse stimulus (EPS). (2) We have fabricated anisotropic three-dimensional microarchitecture with good pore distribution highly aligned morphology enhance cell alignment, fusion myotube formation. With this set up, were able generate fully functional using EPS our doped-biocomposite obtain mature on scale. also characterized distribution, swelling, stiffness conductivity scaffold. Moreover, proved cells viable could fuse in (3D) myotubes throughout In conclusion, biocompatible customizable 3D culture suitable wide range applications such as organ-on-a-chip, drug screening, transplantation disease modelling.