Sequence and organization of the human vitamin D-binding protein gene
作者： Andreas Braun , Andrea Kofler , Susanne Morawietz , Hartwig Cleve
DOI: 10.1016/0167-4781(93)90005-X
关键词: Subcloning 、 Exon 、 Intron 、 Gene 、 Coding region 、 Nucleic acid sequence 、 Genetics 、 Molecular biology 、 Biology 、 Exon shuffling 、 Splice site mutation
摘要: The structure and organization of the human vitamin D-binding protein (DBP) gene has been determined. is composed 13 exons 12 intervening sequences. With help polymerase chain reaction (PCR) introns were amplified using exon-specific oligonucleotide primers, sequenced after subcloning; exon/intron borders 2, 5, 7, 9 10 completely; 1, 3, 4, 6, 8, 11 in part. We designed intron-specific primers for amplification each exon by PCR-method. This permits analysis mutational function-related sites. By comparison with genes albumin alpha-fetoprotein DBP/GC confirmed as a member this multigene family. location coding region DBP-gene identical position rat DBP-gene.
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