Various antibacterial mechanisms of biosynthesized copper oxide nanoparticles against soilborne Ralstonia solanacearum

摘要: The substantial antimicrobial efficacy of nanoparticles against phytopathogens has been extensively investigated for advanced agricultural applications. However, few reports have focused on soilborne pathogenic bacteria. aim this study was to obtain sustainably synthesized copper oxide (CuONPs) using papaya leaf extracts and investigate the bactericidal activity these CuONPs Ralstonia solanacearum, cause bacterial wilt, under laboratory greenhouse conditions. results showed that possessed strong antibacterial all R. solanacearum were killed after exposure 250 μg mL−1 CuONPs. could interact with cells prevent biofilm formation, reduce swarming motility disturb ATP production. Ultrastructural observations by transmission electron microscopy (TEM) revealed interactions CuONPs, suffered significantly from nanomechanical damage cytomembrane, accompanied absorption multiple nanoparticles. In addition, molecular studies identified downregulation mechanism a series genes involving pathogenesis motility. control efficiency in tobacco wilt disease management conditions verified root irrigation application, demonstrating as-prepared reduced occurrence index. Our developing biosynthesized as biocompatible alternative management.