Alcohol metabolism increases the replication of hepatitis C virus and attenuates the antiviral action of interferon
作者： Erin M. McCartney , Ljiljana Semendric , Karla J. Helbig , Susan Hinze , Brett Jones
DOI: 10.1086/593216
关键词: Oxidative stress 、 Interferon 、 Virology 、 Hepacivirus 、 Biology 、 CYP2E1 、 Viral replication 、 Hepatitis C virus 、 Ethanol metabolism 、 Virus
摘要: The interactions between hepatitis C virus (HCV) and alcohol metabolism are not well understood. To determine the effect that has on HCV replication antiviral action of interferon (IFN), Huh-7 cells harbor metabolize ethanol via introduced expression cytochrome P450 2E1 (Cyp2e1) were treated with IFN-alpha. Treatment these (0-100 mmol/L) significantly increased replication. This was dependent Cyp2e1 alcohol-metabolized oxidative stress (OS), because antioxidant N-acetylcysteine blocked this effect. Furthermore, anti-HCV IFN-alpha attenuated in presence metabolism, most likely attenuation Stat1 tyrosine-701 phosphorylation. These vitro results mimic what is often noted clinically, further dissection model system will aid our understanding metabolism.
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