Structure of Ia antigens: identification of dimeric complexes formed by the invariant chain.

摘要: A 2-dimensional gel technique was used for the identification of disulfide-linked polypeptide complexes formed by Ia chains and their corresponding subunits. Several dimers containing invariant (Ii) chain were found. The most prominent one consisted 2 Ii chains. In addition, molecules found to be covalently linked 3 unknown components 41,000, 27,000, 10,000 15,000 m.w. dimeric form exists also after alkylation free SH-groups with iodoacetamide, which suggests that formation dimer is not result experimental conditions. Furthermore, noncovalently associated processed alpha- beta-chains as well precursors alpha p beta could detected. No mixed composed processor observed, indicates processing insertion into membrane take place only dimerization.