Study of Langmuir and Langmuir-Blodgett films of odorant-binding protein/amphiphile for odorant biosensors.

摘要: To make ultrathin films for the fabrication of artificial olfactory systems, odorant biosensors, we have investigated mixed Langmuir and Langmuir-Blodgett odorant-binding protein/amphiphile. Under optimized experimental conditions (phosphate buffer solution, pH 7.5, OBP-1F concentration 4 mg L-1, target pressure 35 mN m(-1)), monolayer at air/water interface is very stable has been efficiently transferred onto gold supports, which were previously functionalized by self-assembled monolayers (SAMs) with 1-octadecanethiol (ODT). Atomic force microscopy electrochemical impedance spectroscopy used to characterize (LB) before after contact a specific molecule, isoamyl acetate. AFM phase images show higher contrast molecule due new structure OBP-1F/ODA LB film. Non-Faradaic (EIS) quantify effect based on electrical properties film, as its resistance strongly decreases from 1.18 M Omega (before contact) 25 k (after contact).