Specific and sensitive measurement of FK506 and its metabolites in blood and urine of liver-graft recipients.
作者： U Christians , F Braun , M Schmidt , N Kosian , H M Schiebel
DOI: 10.1093/CLINCHEM/38.10.2025
关键词: Detection limit 、 Transplantation 、 High-performance liquid chromatography 、 Chemistry 、 Reagent 、 Mass spectrometry 、 Chemical ionization 、 Metabolite 、 Chromatography 、 Quantitative analysis (chemistry)
摘要: A specific and sensitive assay for quantifying the immunosuppressant FK506 its metabolites in blood urine was developed. 32-O-Acetyl synthesized used as internal standard. were purified from samples by solid-liquid extraction injected into a high-performance liquid chromatographic (HPLC) system linked to mass spectrometer (MS) particle-beam interface. The derivatives separated interfering material use of 100 x 4 mm C8 analytical column water/acetonitrile or water/methanol gradient elution; they detected negative chemical ionization with methane reagent gas. limit detection 25 pg standard solution, quantification 250 (extracted 1 mL blood). CV 11.3% at 5 ng, no interferences other drugs found.
oup.com
aaccjnls.org参考文章(17)
H Hatanaka, H Imanaka, M Nishiyama, T Kino, M Okuhara, M Kohsaka, H Aoki, T Goto, Discovery of FK-506, a novel immunosuppressant isolated from Streptomyces tsukubaensis. Transplantation proceedings. ,vol. 19, pp. 4- 8 ,(1987)
A. Krajack, E. M. Cadoff, R. Venkataramanan, S. Todo, Ashokkumar Jain, J. J. Fung, T. E. Starzl, Assay of FK 506 in plasma. Transplantation proceedings. ,vol. 22, pp. 50- 51 ,(1990)
R Venkataramanan, S Todo, TE Starzl, A Jain, J Fung, AJ Demetris, FK 506 for human liver, kidney, and pancreas transplantation ,(1989)
R. Huber, K. Zech, Chapter 2 Determination of Drugs and Their Metabolites in Biological Samples by Fully Automated Hplc with on-line Solid-Liquid Extraction and Pre-Column Switching Journal of chromatography library. ,vol. 39, pp. 81- 143 ,(1988) , 10.1016/S0301-4770(08)60378-4
K. Abuelmagd, T. Zerbe, V. Warty, R. Venkataramanan, S. Todo, A. Jain, A. Zeevi, T. Starzl, J. Fung, G. Eiras, M. Woan, R. J. Duquesnoy, A. J. Demetris, Mario Alessiani, In vitro assessment of FK 506 immunosuppressive activity in transplant patients. Transplantation proceedings. ,vol. 23, pp. 2897- 2899 ,(1991)
Kareem Abu-Elmagd, C. D. Ackerman, S. Todo, A. W. Thomson, D. Van Thiel, J. J. Fung, T. E. Starzl, B. V. Jegasothy, FK 506: a new therapeutic agent for severe recalcitrant psoriasis. Transplantation proceedings. ,vol. 23, pp. 3322- 3324 ,(1991)
M C Friob, Dominique Latinne, Georges Lhoëst, Pierre Wallemacq, Jean-Bernard Otte, A. Hassoun, A combined HPLC-ELISA evaluation of FK 506 in transplant patients. Transplantation proceedings. ,vol. 23, pp. 2750- ,(1991)
Hirokazu Tanaka, Akio Kuroda, Hiroshi Marusawa, Hiroshi Hatanaka, Toru Kino, Toshio Goto, Masashi Hashimoto, Toru Taga, Structure of FK506, a novel immunosuppressant isolated from Streptomyces Journal of the American Chemical Society. ,vol. 109, pp. 5031- 5033 ,(1987) , 10.1021/JA00250A050
Kanji Takada, Mamoru Oh-Hashi, Hiroshi Yoshikawa, Shozo Muranishi, Michihisa Nishiyama, Hiromitsu Yoshida, Takehisa Hata, Hirokazu Tanaka, Determination of a novel potent immunosuppressant (FK-506) in rat serum and lymph by high-performance liquid chromatography with chemiluminescence detection Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 530, pp. 212- 218 ,(1990) , 10.1016/S0378-4347(00)82324-5
Tanaka H, Iwasaki K, Katayama N, Hane K, Tutumi T, Niwa M, Nagase K, Kobayashi M, Tamura K, Nakamura K, FK 506 assay past and present--characteristics of FK 506 ELISA. Transplantation proceedings. ,vol. 23, pp. 2725- 2729 ,(1991)