The Characterization of Intracellular and Extracellular Biomineralization Induced by Synechocystis sp. PCC6803 Cultured under Low Mg/Ca Ratios Conditions

作者: Zuozhen Han , Yanyang Zhao , Huaxiao Yan , Hui Zhao , Mei Han

DOI: 10.1080/01490451.2016.1197986

关键词: Scanning electron microscopeTransmission electron microscopyBiomineralizationPrecipitation (chemistry)Rate-determining stepSelected area diffractionCalciteCrystallographyNuclear chemistryBiologyExtracellular

摘要: ABSTRACTIn order to simulate the precipitation process of microbial limestone at offshore ancient ocean, different calcites induced by Synechocystis sp. PCC6803 in culture media with low Mg/Ca ratios (0.01 M Ca2+, = 0, 0.2, 0.4, 0.6) were investigated, and characteristics intracellular extracellular biomineralization described. Carbonic anhydrase activity medium was further detected. The ultrathin slices cells analyzed transmission electron microscope (TEM) selected area diffraction (SAED). Then precipitates polarizing microscope, scanning (SEM), X-ray (XRD) energy dispersive spectrometer (EDS). results showed that under mainly morphologies. CA accelerated pivotal rate limiting step calcite p...

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