Partial Hepatectomy Induced Long Noncoding RNA Inhibits Hepatocyte Proliferation during Liver Regeneration

摘要: Liver regeneration after partial hepatectomy (PHx) is a complex and well-orchestrated biological process in which synchronized cell proliferation induced response to the loss of liver mass. To define long noncoding RNAs (lncRNAs) that participate regulation regeneration, we performed microarray analysis identified more than 400 lncRNAs exhibiting significantly altered expression. Of these, one lncRNA, LncPHx2 (Long RNA by PHx 2), was highly upregulated during regeneration. Depletion using antisense oligonucleotides led transient increase hepatocyte rapid Gene expression showed depletion resulted upregulation mRNAs encoding proteins known promote proliferation, including MCM components, DNA polymerases, histone proteins, transcription factors. interacts with making it candidate regulate MCMs other genes post-transcriptionally. Collectively, our data demonstrate key lncRNA participates negative feedback loop modulating through RNA-RNA interactions.