Association with beta 2-microglobulin controls the expression of transfected human class I genes.

摘要: The genes encoding HLA-B27K and HLA-B27W were transfected into murine recipient cells. A monoclonal antibody HC-10, directed against free B-locus heavy chain, was the only reagent capable of efficiently detecting HLA-B27 chains in detergent lysates. These devoid sialic acid. Trace amounts could be isolated with anti-HLA-A,-B W6/32, which reacts chain beta 2-microglobulin complex. In marked contrast, HLA-A2 -B7 genes, when transfected, yielded easily detectable antigen precipitable carried usual complement acids. Because alpha 3 domains HLA-B7 more COOH-terminal portions are identical amino acid sequence, structural elements polymorphic 1 2 must control association 2-microglobulin. Introduction a human gene L cells rescued expression at cell surface, as evidenced by reactivity surface staining, presence on chain.