Hybrid proteins with organophosphorus hydrolase activity and fluorescence of deGFP4 protein
作者： D. A. Gudkov , I. V. Lyagin , V. V. Verkhusha , E. N. Efremenko
DOI: 10.3103/S0027131411020064
关键词: Inducer 、 Biochemistry 、 Two-hybrid screening 、 Hydrolysis 、 Enzyme 、 Hydrolase 、 Green fluorescent protein 、 Chemistry 、 Fluorescence 、 Biosynthesis
摘要: New genetic constructs encoding synthesis of hybrid proteins possessing organophosphorus hydrolase activity and properties the pH sensitive analog green fluorescent protein were developed. It was established that 0.1 mM biosynthesis inducer cultivation for 10 h after induction necessary maximum yield in soluble active form E. coli cells. The demonstrated level one new 2- to 25-fold higher than analogs known from literature. found within characteristics (pH optimum, thermal stability catalytic efficiency) different respective native enzyme. shown fluorescence depended on medium a manner similar individual protein. Interrelation OPH manifested itself hydrolysis compounds by example proteins.
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