Study on the Interaction between Isatin-β-Thiosemicarbazone and Calf Thymus DNA by Spectroscopic Techniques.
作者： Parvaneh Pakravan , Shahla Masoudian
关键词: Binding site 、 Chemistry 、 Isatin 、 Crystallography 、 Stereochemistry 、 Spectroscopy 、 Semicarbazone 、 Base pair 、 Stacking 、 Intercalation (chemistry) 、 DNA
摘要: The interaction between isatin-β-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7.4) using Neutral Red (NR) dye as a spectral probe by UV–Vis absorption fluorescence spectroscopy, well viscosity measurements. IBT is stabilized intercalation the (K [IBT –DNA] = 1.03×105 M−1), displaces NR from NR–DNA complex. binding constants Kf number of sites (n≈1) with were obtained fluorescence quenching method at different temperatures. Furthermore, enthalpy entropy reaction CT-DNA showed that enthalpy-favored entropy-disfavored. changes base stacking upon are reflected circular dichroic (CD) studies. increase solution another evidence to indicate that, able be intercalated pairs.
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