Effect of different freezing velocities on the quality and fertilising ability of cryopreserved rabbit spermatozoa.
作者： E. Mocé , E. Blanch , A. Talaván , M. P. Viudes de Castro
DOI: 10.1071/RD14009
关键词: Anatomy 、 Animal science 、 Litter (animal) 、 Reproductive biology 、 Reproductive technology 、 Human fertilization 、 Biology 、 Spermatogenesis 、 Cryopreservation 、 Liquid nitrogen 、 Embryo culture
摘要: The freezing step of the cryopreservation protocol negatively influences quality and fertilising ability rabbit spermatozoa. This study determines effect different rates on spermatozoa cryopreserved with dimethylsulfoxide (DMSO) (1.75M) sucrose (0.05M). Ejaculates from meat line males (n=12) were pooled each pool (n=7) was split into four aliquots. One group straws (control, C) frozen in static liquid nitrogen vapour (5cm above nitrogen, 10min) other groups at (°Cmin(-1)) -6°C to -100°C using a programmable freezer: slow (-15°Cmin(-1), S), medium (-40°Cmin(-1), M) or fast (-60°Cmin(-1), F). After thawing (50°C, 12s), highest (P<0.05) C M samples lowest S F samples. presented litter sizes (P≤0.05) fertility whilst exhibited values. In conclusion, rate affects both frozen-thawed spermatozoa, (-15°Cmin(-1)) (-60°Cmin(-1)) being detrimental for ability.
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sci-hub.se 参考文章(28)
A. Trocino, C. Cortell, S. D. Lukefahr, M. P. Viudes de Castro, G. Xicato, Effect of gelatin addition to freezing extender on rabbit semen parameters and reproductive performance. Proceedings of the 9th World Rabbit Congress, Verona, Italy, 10-13 June 2008. pp. 327- 332 ,(2008)
David T. Melican, Christine A. Cammuso, Stephen M. Blash, William A. Gavin, Cryopreservation of sperm ,(2001)
R. Lavara, J.S. Vicente, M. Baselga, Genetic parameter estimates for semen production traits and growth rate of a paternal rabbit line. Journal of Animal Breeding and Genetics. ,vol. 128, pp. 44- 51 ,(2011) , 10.1111/J.1439-0388.2010.00889.X
R.P. Amann, B.W. Pickett, Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa Journal of Equine Veterinary Science. ,vol. 7, pp. 145- 173 ,(1987) , 10.1016/S0737-0806(87)80025-4
A.M. Petrunkina, R.A.P. Harrison, Systematic misestimation of cell subpopulations by flow cytometry: a mathematical analysis. Theriogenology. ,vol. 73, pp. 839- 847 ,(2010) , 10.1016/J.THERIOGENOLOGY.2009.09.007
Xian-rong Xiong, Li-jun Wang, Yong-sheng Wang, Song Hua, Xiang-dong Zi, Yong Zhang, Different preferences of IVF and SCNT bovine embryos for culture media. Zygote. ,vol. 22, pp. 1- 9 ,(2014) , 10.1017/S0967199412000184
JS Vicente, MP Viudes-de-Castro, A sucrose-DMSO extender for freezing rabbit semen. Reproduction Nutrition Development. ,vol. 36, pp. 485- 492 ,(1996) , 10.1051/RND:19960504
J. Estany, M. Baselga, A. Blasco, J. Camacho, Mixed model methodology for the estimation of genetic response to selection in litter size of rabbits Livestock Production Science. ,vol. 21, pp. 67- 75 ,(1989) , 10.1016/0301-6226(89)90021-3
J. J. Parrish, R. H. Foote, Fertility of Cooled and Frozen Rabbit Sperm Measured by Competitive Fertilization1 Biology of Reproduction. ,vol. 35, pp. 253- 257 ,(1986) , 10.1095/BIOLREPROD35.2.253
Raquel Lavara, Eva Mocé, Felipe Lavara, María Pilar Viudes de Castro, José Salvador Vicente, None, Do parameters of seminal quality correlate with the results of on-farm inseminations in rabbits? Theriogenology. ,vol. 64, pp. 1130- 1141 ,(2005) , 10.1016/J.THERIOGENOLOGY.2005.01.009